Journal: The Journal of Biological Chemistry
Article Title: AKAP2 is required for assembly of cytoskeletal signaling complexes that promote growth and metastasis of triple-negative breast cancer
doi: 10.1016/j.jbc.2026.111329
Figure Lengend Snippet: AKAP2 is upregulated in basal-like triple-negative breast cancer. A , average AKAP2 iBAQ intensity across 20 cell lines organized by subtype. “N” indicates normal-like. Data were reported in Lawrence, et al. . Error bars indicate standard deviation. B , immunoblot analysis of AKAP2 isoform levels and a selected list of EMT markers. Actin served as a loading control. C , densitometric quantification of AKAP2 protein expression normalized to actin. Error bars indicate SEM and statistical significance was determined by ordinary one way ANOVA with Sidak’s multiple comparisons test (∗p adj < 0.05, ∗∗ p adj < 0.01). D , AKAP2 mRNA expression from the METABRIC dataset analyzed through cBioportal. Center lines indicate median and dotted lines indicate quartiles. Statistical significance was determined by Kruskal-Wallis test with Dunn’s multiple comparisons test (∗p adj < 0.05, ∗∗∗∗ p adj < 0.0001). E , immunofluorescent detection of AKAP2 ( green ) in a TNBC patient tumor. Ki67 ( magenta ) positive nuclei ( blue ) were used as a tumor marker. F , immunofluorescent detection of AKAP2 ( green ) of a field of MDA-MB-231 cells co-stained with tubulin ( magenta ) and DAPI ( blue ). G , a higher magnification view of a single MDA-MB-231 cell stained with AKAP2 ( green ), tubulin ( magenta ), and DAPI ( blue ) to visualize subcellular distribution of AKAP2. H , immunofluorescent detection of AKAP2 ( green ) in a MDA-MB-231 cell co-stained with VASP ( magenta ). I , immunoblot analysis of AKAP2 isoform levels in control and shAKAP2 MDA-MB-231 cells. Ponceau S served as a loading control. J , densitometric quantification of AKAP2 isoform expression with expression normalized to Ponceau S and subsequent normalization to shScr untreated control cells. Error bars indicate SEM and statistical significance was determined by two-way ANOVA with Dunnet’s multiple comparisons test (ns: not significant, ∗∗p adj < 0.01). N values indicate biological replicates.
Article Snippet: Slides were incubated with primary antibodies against AKAP2 (1:100, Novus Biological, NB100-61604) and Ki67 (1:1000, Cell Signaling Technology, 9449) in blocking solution overnight at room temperature in a humidity chamber.
Techniques: Standard Deviation, Western Blot, Control, Expressing, Marker, Staining
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